What is an Electron Microscope?
What is an Electron Microscope? – Light microscopes developed into an electron microscope revolutionized for better understanding of structure a shorter length. The resolving power is high that its detail can is up to in, objects can be magnified greatly without loss of clarity. An electron microscope can give a clear picture of an object that is magnified 500 times even 3D images( scanning electron microscope) This allow us to study the submicroscopic structure and provide a better understanding of cytology. Enable the detail of the surface . From the image, we can determine the size and shape of the object.
Give one difference in resolution and magnification in microscopy
Resolution is the ability to distinguish between two separate objects (Separate objects that are close together). It depends on the property of lenses, lens quality and wavelength of illumination. The shorter the wavelength, the better the resolution magnification the number of time the image enlarged than the specimen is. It depends on the combination of lenses used and the power of the lenses. The image is the increase of image but no details.
How Does An Electron Microscope Work
Electron microscopy (EM) a technique used to obtain high-resolution images of biological and non-biological samples. It used in biomedical research to study the detailed structure of tissues, cells, organelles, and complexes of large molecules. The high resolution of EM images is due to the use of electrons (very short wavelengths) as a source of lighting radiation. An electron microscope used with many auxiliary techniques (such as splitting, immunomodulating, negative staining) to answer specific questions. EM images provide basic information about cell function and the structural basis for cell diseases.
Types Of Electron Microscope
There are two main types of electron microscopes – EM (TEM) transport and EM (SEM) scans. Electron microscopy used to monitor the thin samples (tissue sections, molecules, etc.) that electrons can pass through, thus producing an expected image. TEM is similar to conventional (compound) optical microscopes in many ways. TEM especially used for intracellular imaging (thin slices), the structure of protein molecules (in contrast to mineral shades), molecular regulation of cellular structural viruses and capillaries (prepared with negative staining techniques), and protein molecules in the cell membrane arrangement (breaking freezing).
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